We look deeper,
so patients are safer.

Specialist transfusion, immunology and genomic technology that analyses down to the DNA, so every patient receives safer, more precise care.

Explore the portfolio Talk to our team

01
  1. Lumilight
  2. How ECP works
  3. Irradiation
  4. Indications
Lumilight Skip section

01 Cellular therapy

Lumilight.Photopheresis, made in Italy.

Automated, closed and fully traceable photopheresis, in a bench-top device.

Smart where it matters.Simple everywhere else.

  • Automatic dosingAn integrated algorithm calculates irradiation time and 8-MOP volume.
  • Two patient proceduresBuilt-in protocols for low-weight and normal-weight patients.
  • Guided touchscreenOn-screen, step-by-step procedure for a standardised workflow.

Uniform UV-A.Across the entire bag.

  • Real-time HCTIntegrated haematocrit sensor reads every bag.
  • Functionally closedLLK-01 kits with an integrated antibacterial filter, ready for tube-to-tube welding.
  • Full sensor setHCT, pressure, temperature, leakage, UV-A emission and LED status, with acoustic and visual alarms.

Closed to the operator.Traceable for every patient.

  • Full traceabilityRFID, data recording and export to common management systems.
  • Two security levelsThe operator is recorded on every treatment.
PATIENTLEUKAPHERESIS018-MOP02UV-A03 · LumilightREINFUSION04

How offline photopheresis works

One closed loop, from the patient back to the patient.

Cells collected on a cell separator are connected to the Lumilight kit by sterile tube welding, treated, and returned.

  1. A cell separator draws the patient's blood and collects the mononuclear cell fraction. Red cells and plasma go straight back to the patient.

  2. 8-methoxypsoralen (8-MOP) is added to the collected cells. Lumilight reads the haematocrit in real time and calculates the exact 8-MOP volume.

  3. LED UV-A activates the 8-MOP inside the treated leukocytes. The integrated algorithm sets the energy and irradiation time for a standardised dose. This is the step Lumilight performs.

  4. The treated cells are reinfused into the patient. More than 95% of them undergo apoptosis within 72 hours.

  5. Antigen-presenting cells take up the apoptotic cells, which is thought to promote regulatory T cells and tolerance rather than broad immunosuppression.

Inside every treatment

One bag, one thin layer.
The same dose for every cell.

>95%apoptosis after 72 hours
15 minaverage treatment time
365 nmLED UV-A, homogeneous across the bag
The treatment bag lying flat in the Lumilight tray, filled with the patient's cells The same bag under 365 nm UV-A light Before 365 nm UV-A
Uniform across the whole bag. The kit lies flat in the tray under a clear lid, so every cell receives the same UV‑A dose.

Indications

Established in haemato-oncology, transplantation and autoimmune disease.

ECP applications according to the latest ASFA guidelines, as listed by Pelham Crescent.

AHaemato-oncology

  • Graft-versus-host disease (GvHD)
  • Cutaneous T-cell lymphoma (CTCL)

BSolid-organ transplantation

  • Heart: cellular and recurrent rejection, rejection prophylaxis
  • Lung: bronchiolitis obliterans syndrome
  • Liver: acute rejection, prophylaxis, calcineurin-inhibitor delay

CAutoimmune and dermatology

  • Systemic sclerosis (scleroderma)
  • Pemphigus vulgaris
  • Psoriasis
  • Recalcitrant atopic dermatitis
  • Nephrogenic systemic fibrosis

DGastroenterology

  • Inflammatory bowel disease

02 Rapid genotyping

FluoQube.One platform. Every genotype.

Real-time PCR genotyping for HLA, HPA and red cell antigens, with ready-to-use FluoGene kits.

Open. Load. Run.Three minutes hands-on.

  • Ready-to-use kitsA FluoGene kit for each test system, from a single HLA locus to a full match.
  • Results in under an hourAutomated genotyping by real-time PCR.
  • One systemThe same instrument and method for HLA, HPA and red cell typing.

Barcoded. Sealed.Nothing gets mixed up.

  • Barcoded testsEvery FluoGene plate carries a unique barcode, so assays cannot be mixed up.
  • Closed systemPrevents contamination.
  • Multiplex PCRUp to four fluorescence channels in each reaction.

Every well, read.Every result, calculated.

  • FluoGene softwareqPCR data is imported automatically and the typing result is calculated.
  • Two-step validationGDP-compliant result validation, with printed reports.
  • LIMS-compatible exportResults move on without retyping, including HML files.

The workflow

From blood to result
in under two hours.

The same five steps for HLA, HPA and red cell typing. Most of the time is the machine's, not yours.

  1. Blood sample

    The starting material: a whole blood sample, taken in the usual way.

  2. DNA isolation

    40 min

    BEXS 12 extracts the DNA of up to twelve samples in parallel.

  3. PCR set-up

    < 5 min

    Ready-to-use kits: the barcoded plate is loaded and sealed. About 3 minutes of hands-on time.

  4. Real-time PCR

    < 1 h

    FluoQube runs the plate. The raw data is imported automatically.

  5. Result

    The FluoGene software calculates the genotype and prints or exports the report.

Why FluoGene

Quick to run.
Hard to get wrong.

  • 3minEasy and fast

    Ready-to-use kits, 3 minutes of hands-on time and automated genotyping in under one hour.

  • 11lociFlexible

    HLA typing in 96-well or 384-well format, from a single locus up to all eleven loci, for one or two patients.

  • 2×duplicateBuilt for safety

    A closed system limits contamination. Barcoded plates give clear traceability, qPCR data transfers automatically, and safety reactions run in duplicate for every specificity.

  • 1systemMatched as a system

    FluoQube, the kits and the software are tuned to each other, backed by a specialised support team, webinars and workshops.

The FluoGene range

Three families.
One platform.

  1. From a single locus to the full match: HLA-A, B, C, DRB1, DRB3/4/5, DQA1, DQB1, DPA1 and DPB1, with the Match kits typing all eleven loci including null alleles.

    • A
    • B
    • C
    • DRB1
    • DRB3/4/5
    • DQA1
    • DQB1
    • DPA1
    • DPB1
    • B27

  2. Platelet antigens for compatible transfusions and FNAIT risk: eight HPA systems in one run, or a 12-sample HPA-1a/b screen.

    • HPA-1
    • -2
    • -3
    • -4
    • -5
    • -6
    • -9
    • -15

  3. From ABO and RhD variants to rare blood group antigens, for donor and patient typing.

    • ABO
    • RhD
    • RhCE
    • Kell
    • Kidd
    • Duffy
    • MNS
    • Dombrock
    • Lutheran
    • Diego
    • Colton
    • Knops
    • Yt
    • LW

The range

Every kit,
what it detects.

Tests per plate and tests per kit, from the inno-train kit list.

HLA14 kits

TaqMan-based typing of the HLA class I and class II loci, from a single locus to all eleven. The Match kits type the full set in one go, in 96-well or 384-well format.

  • HLA · 01 / 14HLA-FluoGeneA

    1 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 02 / 14HLA-FluoGeneB

    1 of 9 loci

    Tests per plate
    1 / 2
    Tests per kit
    10 / 20
  • HLA · 03 / 14HLA-FluoGeneC

    1 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 04 / 14HLA-FluoGeneABC

    3 of 9 loci

    HLA class I alleles

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 05 / 14HLA-FluoGeneABDR

    4 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 06 / 14HLA-FluoGeneDR

    2 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 07 / 14HLA-FluoGeneDRDQ

    3 of 9 loci

    Tests per plate
    1 / 3
    Tests per kit
    10
  • HLA · 08 / 14HLA-FluoGeneDRDQ plus

    4 of 9 loci

    Upon request only.

    Tests per plate
    2
    Tests per kit
    20
  • HLA · 09 / 14HLA-FluoGeneDRDQDP plus

    6 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 10 / 14HLA-FluoGeneNX ABCDRDQ

    7 of 9 loci

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 11 / 14HLA-FluoGeneNX Match

    9 of 9 loci

    96-well

    All eleven loci in SABR resolution. Detects all 23 CWD null alleles and the CIWD common and intermediate null alleles.

    Tests per plate
    1 (2 plates per test)
    Tests per kit
    5
  • HLA · 12 / 14HLA-FluoGene384 Match

    9 of 9 loci

    384-well

    All eleven loci in SABR resolution, for the 384-well block. Detects all 23 CWD null alleles and the CIWD common and intermediate null alleles.

    Tests per plate
    1 / 2
    Tests per kit
    10
  • HLA · 13 / 14HLA-FluoGeneDPB1

    1 of 9 loci

    Upon request only.

    Tests per plate
    1
    Tests per kit
    10
  • HLA · 14 / 14HLA-FluoGeneB27

    1 of 9 loci

    HLA-B*27 alleles associated with ankylosing spondylitis (Morbus Bechterew). A version for any real-time PCR cycler, B27 Q, is available (002 078 00Q, 100 tests per kit).

    Tests per plate
    12
    Tests per kit
    48

FluoGene software

From raw curves
to a typing result.

  • Automatic import

    Raw data flows into the software by the plate barcode, so assays cannot be mixed up and nothing is transcribed by hand.

  • Clear results

    All eleven HLA loci at a glance, or every blood group with genotypes and predicted phenotypes in ISBT notation. Filters by allele category and frequency, RHD zygosity and allele selection help the reading.

  • A traceable record

    Two-step validation, lot and plate information, printable reports, and an export to LIMS. HLA results can also be exported as an HML file for Eurotransplant.

26 ready-to-use kits.
One instrument.

14HLA-FluoGene kits
2HPA-FluoGene kits
10RBC-FluoGene kits

IVDR-CE marked HLA-FluoGene kits, FluoGene software and FluoQube.

03 NGS genotyping

NGStype.Genotyping, read by read.

High-resolution HLA and blood group genotyping by next-generation sequencing, on Illumina and Oxford Nanopore sequencers.

Amplify. Pool. Sequence.One workflow, any run size.

  • Long-range PCR kitPrimers and master mix for 11 HLA loci in the box.
  • Your library preparationNEBNext for Illumina, the Rapid Barcoding Kit for Nanopore.
  • Run size to fitFrom one sample on a MinION to 440 on a MiSeq V3.

Every read.Every base.

  • Any FASTQ fileImport the run from your sequencer into NGStype software.
  • ISBT-based databaseBlood group results, developed with Prof. Dr. Christoph Gassner.
  • Heterozygous positions flaggedIn the coverage plot, with the ratio of each base.

Two alleles.Told apart.

  • Short and long readsDifferent algorithms for each, in the same software.
  • High-resolution resultsEleven HLA loci and the main blood group systems.
  • Report for every locusCoverage and quality for each exon and intron.

Part A of 2

HLA-NGStype.
High-resolution typing.

Eleven HLA loci from one long-range PCR kit, sequenced on Illumina or Oxford Nanopore instruments.

HLA-SuBiTo NGStype

Your sequencer.
Your way.

The same long-range PCR kit feeds two workflows. Choose the one that fits your samples and your instrument.

Amplify. Pool.Once, for both.

  • 01Long-range PCRPrimers and master mix for eleven HLA loci are in the box.
  • 02Pooling and purificationThe amplicons of each sample are pooled and cleaned up.

Then choose.Two ways to sequence.

  • AIlluminaSix hours from sample to sequencer. Many samples in parallel.
  • BOxford NanoporeFive hours from sample to sequencer. One sample or more.

Illumina.Many samples, high quality.

  • 03Fragmentation, end preparation, adaptor ligation
  • 04Size selection
  • 05Final PCR and purification
  • 06Run on the sequencerOvernight.

High data quality (>Q30), low price per sample with many samples. With NEBNext UltraExpress DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina (NEB).

Oxford Nanopore.Fast, from one sample.

  • 03Barcode ligation
  • 04Pooling, purification, adapter ligation
  • 05Run on the sequencerMinION, FLO-MIN114.

Fast and easy workflow, phased haplotypes, little initial cost. With the Rapid Barcoding Kit 24 V14 (ONT).

One analysis.Whichever you chose.

  • →Any FASTQ fileImport the run from your sequencer into the NGStype software.
  • →Short and long readsDifferent algorithms for each, in the same software.

Eleven loci.Every exon, end to end.

  • →5′ UTR to 3′ UTRHLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1 and -DRB1/3/4/5, each amplified in full.
  • →Locus reportCoverage and quality for each exon and intron, the ratio at each heterozygous position, and a summary for all loci of a sample.

HLA-SuBiTo NGStype

Your sequencer.
Your way.

The same long-range PCR kit feeds two workflows. Choose the one that fits your samples and your instrument.

  1. 01Long-range PCR
  2. 02Pooling and purification

Illumina

6 hsample to sequencer

  1. 03Fragmentation, end preparation, adaptor ligation
  2. 04Size selection
  3. 05Final PCR and purification
Illumina MiSeq sequencer

Run on the sequencerOvernight

Samples per run

  • 20MiSeq V2 Nano
  • 56iSeq 100 i1
  • 70MiSeq V2 Micro
  • 140MiniSeq High-Output
  • 440MiSeq V3
  • High data quality (>Q30)
  • Many samples in parallel
  • Low price per sample with many samples

With NEBNext UltraExpress DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina (NEB).

Oxford Nanopore

5 hsample to sequencer

  1. 03Barcode ligation
  2. 04Pooling, purification, adapter ligation
Oxford Nanopore MinION sequencer

Run on the sequencerMinION

MinION · FLO-MIN114

1sample in 20 minutes
3samples per hour
  • Fast and easy workflow
  • NGS with one sample or more
  • Phased haplotypes
  • Little initial cost

With the Rapid Barcoding Kit 24 V14 (ONT).

Data analysisNGStype software

More workflow options: the inno-train NGStype library preparation kit (coming soon), your own library preparation, other Oxford Nanopore kits and MGI sequencers.

Coverage

Eleven HLA loci.
One kit.

HLA-A, -B, -C, -DPA1, -DPB1, -DQA1, -DQB1 and -DRB1/3/4/5, amplified from the 5′ UTR to the 3′ UTR of each amplicon.

11HLA loci
24samples per kit
440samples on one MiSeq V3 run
Amplified exons and UTRs per locus
Class I
  1. HLA-A5′123456783′3.4 kb
  2. HLA-B5′12345673′4.3 kb
  3. HLA-C5′123456783′4.4 kb
Class II
  1. HLA-DPA15′12343′5.5 kb
  2. HLA-DPB15′123453′7.4 kb
  3. HLA-DQA15′12343′5.9 kb
  4. HLA-DQB15′1234563′5.9 kb
  5. HLA-DRB15′1234563′5.9 kb
  6. HLA-DRB35′1234563′5.6 kb
  7. HLA-DRB45′1234563′5.1 kb
  8. HLA-DRB55′1234563′4.9 kb

NGStype software

Any FASTQ in.
A typing result out.

  • Short and long readsImport any FASTQ file. Different algorithms for short and long reads.
  • Coverage plotWith indicators for heterozygous positions and contigs, and the quantity of each individual nucleotide.
  • Locus reportCoverage and quality for each exon and intron, the ratio at each heterozygous position, and a summary report for all loci of a sample.
NGStype software: coverage plot and nucleotide view of one exon
NGStype software: locus report

Part B of 2

RBC-NGStype.
Red cell antigens, sequenced.

Blood group genotyping by next-generation sequencing, from ABO and Rh to the rarer systems.

ONE DAY AT THE BENCHOVERNIGHTDNA SAMPLEMULTIPLEX PCRPOOLADAPTORSFINAL PCRILLUMINA RUNany Illumina systemRESULTNGStype software

How RBC-NGStype works

One day at the bench.
The run goes overnight.

Twenty-three blood group systems in three kits, many samples in parallel, on any Illumina system.

  1. Each sample's DNA goes into multiplex PCRs, several blood group genes in each tube. CORE covers its six systems in four multiplexes; enCORE and enCORE+ use two each.

  2. The PCR products of every sample go into one pool and are cleaned up together.

  3. End preparation and adaptor ligation turn the pooled amplicons into a library the sequencer can read.

  4. One last PCR and clean-up, and the library is ready to load.

  5. After the final preparation, the run goes onto any Illumina system at the end of the day. Paired-end reads of 2×150 bp; 48 samples fit on a MiSeq Nano flow cell.

  6. The NGStype software, with an ISBT-based blood group database developed with Prof. Dr. Christoph Gassner, reports genotypes and predicted phenotypes for each sample.

What it reads

Twenty-three systems,
around one red cell.

CORE types the six systems most labs need every day. enCORE and enCORE+ add seventeen more, down to the rarer antigens.

Genotype in.Phenotype out.

  • →Every system, one lineThe genotype with its alleles, and the phenotype it predicts.
  • →ISBT allele namesNamed after the ISBT nomenclature.

Three kits.Twenty-three systems.

  • →COREABO, Rh, MNS, Kell, Kidd and Duffy. Available.
  • →enCORESix further systems. Available.
  • →enCORE+Eleven further systems. Coming soon.

Genotype in, phenotype out

Results come in the NGStype software, with an ISBT-based blood group database developed with Prof. Dr. Christoph Gassner: genotypes and predicted phenotypes for each sample.

SystemGenotypePredicted phenotype
ABOABO*A1.01/ABO*O.01.01A
RHRHD*01 · RHCE*01/RHCE*02D+ C+ c+ E− e+
MNSGYPA*01/GYPA*02 · GYPB*03/GYPB*04M+ N+ S+ s+
KELKEL*02/KEL*02K− k+
JKJK*01/JK*02Jk(a+b+)
FYFY*01/FY*02Fy(a+b+)

Example result for one sample, illustration.

Three kits

CORE

001 110 024

4 multiplexes · available

  • ABO
  • Rh
  • MNS
  • Kell
  • Kidd
  • Duffy

enCORE

001 120 024

2 multiplexes · available

  • Lutheran
  • Yt
  • Dombrock
  • Colton
  • Landsteiner-Wiener
  • Knops

enCORE+

001 130 024

2 multiplexes · coming soon

  • P1PK
  • Diego
  • Scianna
  • Chido/Rodgers
  • Gerbich
  • Cromer
  • Indian
  • John Milton Hagen
  • Rh-associated glycoprotein
  • JR
  • LAN

All exons and relevant non-coding region amplifiedKey exons and relevant non-coding region amplified

04 SSP-PCR genotyping

Ready Gene.When serology is not enough.

A weak D reaction. A patient transfused last month. A positive DAT. A rare antigen with no antiserum on the shelf. Ready Gene types these samples from DNA, by SSP-PCR on the thermal cycler and gel you already have.

The answeris in the DNA.

  • Weak D, resolvedD weak and D weak Screen identify the weak D type; the screen finds types 1, 2 and 3 in four reactions.
  • Unaffected by transfusionDonor cells in the patient's sample do not change the genotype.
  • No antiserum neededRare ID and vERYfy type Kpa/Kpb, Dombrock, Lutheran, Diego, Colton, Knops and more.

A band onlyon a perfect match.

  • Sequence-specific primersEach well tests for one allele. The DNA is copied only if the primer's last base matches it.
  • A control in every wellSo a missing band means no match, not a failed reaction: the HGH gene in RBC-Ready Gene, controls of different sizes in HLA-Ready Gene.
  • An integrated negative controlA reaction without DNA, to show the run is clean.

From DNA to resultwithin 90 minutes.

  • Dried, pre-aliquoted primersColour-coded plates, blocks and strips with the primers already in the wells: add DNA and mix.
  • Equipment you already haveA standard thermal cycler and agarose gel electrophoresis.
  • Low DNA inputFor example 320 ng for ABO typing.

Read the bands.Close the case.

  • Visual evaluationRBC-Ready Gene needs no software for interpretation.
  • Ready Gene OnlineBrowser-based evaluation: mark the positive bands with a click, with a tolerance display.
  • No installationNo patient data is stored on inno-train servers.

Ready Gene kits

Start from the question.
Find the kit.

Blood group, HLA and KIR kits share one PCR programme and one way of working, so they can be combined in one run. Every kit is colour coded, with the primers dried in the wells.

Blood groups

A weak D reaction

Start with D weak Screen: four reactions find weak D types 1, 2 and 3. D weak then resolves further weak D types by their SNPs.

  • RBC-Ready Gene

    D weak Screen

    4 wells per test·24 tests per kit, green

    Weak D types 1, 2 and 3

  • RBC-Ready Gene

    D weak

    12 wells per test·12 tests per kit, green

    RHD variants by SNPs, weak D types

Blood groups

A partial or variant D, or an Rh variant

CDE reads the RHD exon pattern together with RHCE. D Add On adds RHD psi, null alleles and RHD*DEL; RHCE variants resolves the RHCE side.

  • RBC-Ready Gene

    CDE

    16 wells per test·12 tests per kit, blue

    RHD exon pattern and variants, RHD negative and psi; RHCE C, c, E, e and Cw

  • RBC-Ready Gene

    D Add On

    8 wells per test·12 tests per kit, blue

    RHD psi, RHD null alleles by SNPs, RHD*DEL

  • RBC-Ready Gene

    RHCE variants

    16 wells per test·12 tests per kit, blue

    RHCE variants by SNPs

Blood groups

RHD zygosity

Zygo-Fast tells RHD homozygous from hemizygous in four reactions, for example for the partner in a pregnancy with anti-D.

  • RBC-Ready Gene

    Zygo-Fast

    4 wells per test·12 tests per kit, colourless

    RHD*01 and RHD negative: RHD zygosity

Blood groups

A transfused patient who needs matched blood

Patients on regular transfusion, as in thalassaemia, need antigen-matched units. Their genotype comes from their own DNA, so donor cells do not interfere. vERYfy covers RHD, RHCE, Kell, Kidd, Duffy, MNS and Dombrock in one run.

  • RBC-Ready Gene

    vERYfy

    24 wells per test·12 tests per kit, purple

    RHD exons 3, 5, 10, RHCE, Kell, Kidd, Duffy, MNS and Dombrock in one run

  • RBC-Ready Gene

    KKD

    8 wells per test·12 tests per kit, yellow

    Kell, Kidd and Duffy

  • RBC-Ready Gene

    JK plus FY

    8 wells per test·12 tests per kit, yellow

    Kidd, Kidd null alleles and Duffy

  • RBC-Ready Gene

    MNS

    7 wells per test·12 tests per kit, purple

    GYPA, GYPB, Vw, Mg, Mur, Hil, Bun and HF

Blood groups

A positive DAT

IgG-coated cells make typing antisera unreliable. DNA is not affected, so the antigens can be typed from the genotype.

  • RBC-Ready Gene

    vERYfy

    24 wells per test·12 tests per kit, purple

    RHD exons 3, 5, 10, RHCE, Kell, Kidd, Duffy, MNS and Dombrock in one run

  • RBC-Ready Gene

    CDE

    16 wells per test·12 tests per kit, blue

    RHD exon pattern and variants, RHD negative and psi; RHCE C, c, E, e and Cw

  • RBC-Ready Gene

    KKD

    8 wells per test·12 tests per kit, yellow

    Kell, Kidd and Duffy

Blood groups

A rare antigen with no antiserum

Rare ID types antigens that are hard to find antisera for: Kpa/Kpb, Dombrock, Lutheran, Cartwright, Diego, Colton, Knops and Landsteiner-Wiener.

  • RBC-Ready Gene

    Rare ID

    16 wells per test·12 tests per kit, colourless

    Kpa/Kpb, Dombrock, Lutheran, Cartwright, Diego, Colton, Knops, Landsteiner-Wiener

  • RBC-Ready Gene

    KEL plus

    8 wells per test·12 tests per kit, yellow

    Kell, Kpa/Kpb, Jsa/Jsb, Kidd and Duffy

Blood groups

An ABO discrepancy

ABO reads the common alleles; ABO Subtype adds the A, B and O variants behind weak or unexpected reactions.

  • RBC-Ready Gene

    ABO

    8 wells per test·12 tests per kit, red

    A1, A2, B, O1 and O2 alleles

  • RBC-Ready Gene

    ABO Subtype

    16 wells per test·12 tests per kit, red

    A1, A2, B, O1 and O2, plus A, B and O variants

HLA

HLA typing, locus by locus

One kit per locus, for transplant work-ups and confirmations.

  • HLA-Ready Gene

    HLA-A

    24 wells per test·40 tests per kit, green

    HLA-A alleles

  • HLA-Ready Gene

    HLA-B

    48 wells per test·20 tests per kit, transparent

    HLA-B alleles

  • HLA-Ready Gene

    HLA-C

    24 wells per test·40 tests per kit, yellow

    HLA-C alleles

  • HLA-Ready Gene

    HLA-DR

    24 wells per test·40 tests per kit, red

    HLA-DRB1 and DRB3/4/5

  • HLA-Ready Gene

    HLA-DQ

    8 wells per test·48 tests per kit, blue

    HLA-DQB1

  • HLA-Ready Gene

    HLA-DPB1

    48 wells per test·20 tests per kit, blue

    HLA-DPB1 alleles

HLA

HLA typing on one plate

Several loci in one run on a 96- or 32-well layout.

  • HLA-Ready Gene

    HLA-ABC

    96 wells per test·10 tests per kit, blue

    Class I: HLA-A, -B and -C on one plate

    Also available with Taq included

  • HLA-Ready Gene

    HLA-ABDR

    96 wells per test·10 tests per kit, transparent

    HLA-A, -B and -DR on one plate

    Also available with Taq included

  • HLA-Ready Gene

    HLA-DRDQ

    32 wells per test·30 tests per kit, transparent

    Class II: HLA-DR and -DQ together

HLA

HLA-B27

Exclusion testing for HLA-B27 in suspected ankylosing spondylitis, in one PCR reaction.

  • Disease association

    HLA-B27

    1 reaction per test·32 / 96 tests per kit, yellow

    B*27:02, *27:04, *27:05, *27:14; and the questionable B*27:06, *27:07, *27:09

    Base pair marker, Axi-Taq and control DNA included

HLA

Before abacavir

HLA-B*57:01 is linked to hypersensitivity to abacavir in HIV treatment. One reaction.

  • Disease association

    HLA-B57

    1 reaction per test·32 tests per kit, green

    HLA-B*57:01, with a probability above 99 % in one reaction

    Base pair marker, Axi-Taq and control DNA included

HLA

Coeliac disease

Two multiplex reactions exclude the DQ2 and DQ8 risk alleles.

  • Disease association

    Coeliac disease

    2 reactions per test·32 tests per kit, blue and red

    HLA-DQA1*05:01, *05:05 and DQB1*02:01, *02:02, *03:02 in two multiplex reactions

    Base pair marker, Axi-Taq and control DNA included

Research

KIR genotyping

For transplantation, autoimmunity, infection and pregnancy research. 24 reactions per test.

  • Research use only

    KIR-Ready Gene

    24 wells per test·16 tests per kit, transparent

    All 15 KIR genes with their known allelic variants

    For research use only

Ready Gene Online: kit selection, gel interface and tolerance display

Ready Gene Online

Evaluate in
your browser.

  • Click the positive bandsA configurable gel interface. The tolerance display shows probable tolerance reactions.
  • Virtual allelesSelect alleles and see the reaction pattern positions directly in the gel.
  • Secure by designNo installation or maintenance. No patient data on inno-train servers; https with SSL encryption.

05 HLA serology

Ready Plate.Will this serum kill these cells?

Before a transplant, the lab has to know whether the recipient carries antibodies against the donor's HLA. The complement-dependent cytotoxicity (CDC) test shows it under the microscope. Ready Plate brings the same reaction to HLA typing, with the antisera already in the wells.

Separatethe lymphocytes.

  • LymphodexBlood with Hanks solution over Lymphodex (density 1.077), 1000 g for 20 minutes. The lymphocytes collect in one ring.

Washand count.

  • 2,000 to 3,000 per µlWashed in Hanks solution or RPMI 1640, then adjusted.

Add the cellsto the tray.

  • Thaw and useThe antisera are already in the wells. Thaw the tray, add 1 µl of cells to each well.
  • 30 minutesAn antibody in the serum binds only the HLA antigen it recognises (DR/DQ: 60 minutes).

Complementdoes the rest.

  • Rabbit complement5 to 6 µl per well, 60 minutes (DR/DQ: 120). It opens only the cells an antibody has marked. Five vials come with every kit.
  • Complement you can checkAn activity test by chess board titration.

Stainand fix.

  • Eosin, then formaldehyde3 to 4 µl eosin 5 % for five minutes, then 5 to 6 µl formaldehyde 37 %. Living cells keep the stain out.

Readthe score.

  • Inverted phase microscopeLysed cells look larger, red and irregular. 0 – 10 % lysed scores 1, negative; 81 – 100 % scores 8, strongly positive.
  • ControlsAnti-HLA positive and negative control sera.

Antiseraalready in the wells.

  • Thaw and useThe Ready Plate arrives with the antisera in the wells. Thaw the tray and the test is ready.
  • Ten traysFrom HLA class I to B27, with 72 or 144 antisera per test.

Cells in,then wait.

  • LymphodexLymphocytes separated over Lymphodex (density 1.077), washed and adjusted to 2,000 to 3,000 per µl.
  • 1 µl per wellAn antibody in the serum binds only the HLA antigen it recognises. 30 minutes (DR/DQ: 60).

Complementdoes the rest.

  • Rabbit complement5 to 6 µl per well, 60 minutes (DR/DQ: 120). It opens only the cells an antibody has marked. Five vials come with every kit.
  • Complement you can checkAn activity test by chess board titration.

Stain, fixand read the score.

  • Eosin, then formaldehydeLiving cells keep the stain out; lysed cells look larger, red and irregular under an inverted phase microscope.
  • Scored 1 to 80 to 10 % lysed scores 1, negative; 81 to 100 % scores 8, strongly positive.
  • ControlsAnti-HLA positive and negative control sera.

HLA-Ready Plate

Ten trays.
Class I to B27.

HLA-Ready Plate ABC 144

Antisera per test
144
Trays per test
2
Typings per kit
5
Article no.
002 011 144

Every kit comes with lyophilised rabbit complement, 5 × 1 ml. Class I antisera lists on request.

  • ABC 144 HLA-A, B, C · 2 trays of 72 antisera per test · 5 typings · 002 011 144
  • ABC 120 HLA-A, B, C · 2 trays of 60 antisera per test · 5 typings · 002 011 120
  • ABC 72 HLA-A, B, C · 1 tray of 72 antisera per test · 10 typings · 002 012 072
  • AB 144 HLA-A, B · 2 trays of 72 antisera per test · 5 typings · 002 010 144
  • AB 120 HLA-A, B · 2 trays of 60 antisera per test · 5 typings · 002 010 120
  • AB 72 HLA-A, B · 1 tray of 72 antisera per test · 10 typings · 002 014 072
  • DR 72 HLA-DR · 1 tray of 72 antisera per test · 10 typings · 002 013 072
  • B27/1 HLA-B27 · 12 antisera, 1 person per tray · 10 typings · 002 021 001
  • B27/2 HLA-B27 · 2 × 12 antisera, 2 persons per tray · 20 typings · 002 022 002
  • B27/5 HLA-B27 · 5 × 12 antisera, 5 persons per tray · 50 typings · 002 023 005

HLA-Ready Plate is made by inno-train Diagnostik GmbH, Kronberg, Germany.

A gel card read after DaraEx: one column positive, the others negative

06 Immunohaematology reagents

DaraEx.Anti-CD38 interference, switched off.

A patient on daratumumab needs blood. Every panel cell reacts, and a real alloantibody could be hiding underneath. DaraEx masks CD38 on the test cells, so screening, identification and crossmatch read true again.

The drug is on the cells.So every test looks positive.

  • CD38 on every red cellThe drug binds it on the reagent cells, and the antiglobulin links them.
  • Daratumumab, isatuximab, felzartamabAnd mezagitamab, with DaraEx.
  • Interference for monthsIt can last up to 6 months after the last dose of the drug.

Cover CD38 first.The drug finds nothing to bind.

  • A Fab fragmentOf an anti-CD38 antibody, with no human Fc region, masking CD38 on the red cell surface.
  • No DTT side effectsNo destruction of Kel antigens, no haemolysis.
  • Nothing else affectedOther antigens and alloantibody reactions stay as they are.

One express protocol.Every test.

  • 0.2 volume per volume of cellsFor 50 µl of 0.8 % cells, add 10 µl of DaraEx.
  • No pre-incubationAdd it directly to the test cells in the gel card.
  • Standard gel cardsBio-Rad ID System and QuidelOrtho MTS.

The panel reads true.The real antibody shows.

  • Alloantibodies stay visibleAnti-K too, which DTT-treated cells would miss.
  • Screening, identification, crossmatchThe same protocol for all three, without pre-selected donor units.
  • A control for the maskingTreated cells must not react with the anti-CD38 control.
DaraEx vial
DaraEx plus vial
Dara-PC vial

DaraEx

DaraEx plus

Dara-PC

The range

Three vials.
One protocol.

Two reagents that mask CD38 on the test cells, and a control that proves the masking worked.

30tests per 300 µl vial
15months shelf life
0minutes of pre-incubation
Masking reagent

DaraEx

The masking reagent

A Fab fragment of an anti-CD38 antibody. Added to the test cells, it covers CD38 so the patient's anti-CD38 drug has nothing to bind. Also inhibits mezagitamab.

  • 300 µl, about 30 tests
  • Purple lid
  • 2 to 8 °C, do not freeze
Masking reagent

DaraEx plus

The masking reagent for routine use

The same antigen masking, made for the routine IAT. It inhibits the anti-CD38 agglutination without side effects on the other blood group antigens.

  • 300 µl, about 30 tests
  • Purple lid
  • 2 to 8 °C
Control

Dara-PC

The anti-CD38 control

An anti-CD38 antibody at 0.5 mg/ml, used in place of the sample. Untreated cells react with it; DaraEx-treated cells must not, which shows the masking worked.

  • 450 µl
  • Blue lid
  • Positive and process control

Protocol

Treat the cells.
Run the card.

Express 0.8 % cells

50 µl cells + 10 µl DaraEx

1 volume of cells + 0.2 volume of DaraEx. Use at once, in the card or in a separate vessel.

Alternative 1.6 % cells

25 µl cells + 25 µl DaraEx

1 volume of cells + 1 volume of DaraEx, final 0.8 %. If the express protocol is not satisfactory.

Control every run

Dara-PC in place of the sample

Treated cells must not react with it. Then evaluate the card as if no anti-CD38 antibody were present.

The range

Three vials.
One protocol.

Two reagents that mask CD38 on the test cells, and a control that proves the masking worked.

  • DaraEx vial, purple lid
    Masking reagent

    DaraEx

    The masking reagent

    A Fab fragment of an anti-CD38 antibody. Added to the test cells, it covers CD38 so the patient's anti-CD38 drug has nothing to bind. Also inhibits mezagitamab.

    • 300 µl, about 30 tests
    • Purple lid
    • 2 to 8 °C, do not freeze
  • DaraEx plus vial, purple lid
    Masking reagent

    DaraEx plus

    The masking reagent for routine use

    The same antigen masking, made for the routine IAT. It inhibits the anti-CD38 agglutination without side effects on the other blood group antigens.

    • 300 µl, about 30 tests
    • Purple lid
    • 2 to 8 °C
  • Dara-PC anti-CD38 control vial, blue lid
    Control

    Dara-PC

    The anti-CD38 control

    An anti-CD38 antibody at 0.5 mg/ml, used in place of the sample. Untreated cells react with it; DaraEx-treated cells must not, which shows the masking worked.

    • 450 µl
    • Blue lid
    • Positive and process control
30tests per 300 µl vial
15months shelf life
0minutes of pre-incubation

Protocol

Treat the cells.
Run the card.

Treat the test cells with DaraEx before the sample or control is added. Then use the cells in the IAT as the card manufacturer describes, and evaluate as if no anti-CD38 antibody were present.

ProtocolCell concentrationTreatmentExample
Express0.8 %1 volume of cells + 0.2 volume of DaraEx. Use at once, in the card or in a separate vessel.50 µl cells + 10 µl DaraEx
Alternative1.6 %1 volume of cells + 1 volume of DaraEx (final 0.8 %). Use if the express protocol is not satisfactory.25 µl cells + 25 µl DaraEx

Include a process control with every run. If a treated cell is still agglutinated by the sample, test it with the control or repeat with the alternative protocol.

Why DaraEx

Faster than DTT.
Gentler on the cells.

  • Easy to implementAn express protocol that fits routine serology, with no pre-incubation of the cells.
  • Blood group antigens stay intactNo destruction of Kel antigens and no haemolysis, unlike standard DTT treatment.
  • No expensive blockersNo anti-idiotypic antibodies and no soluble CD38 protein needed.

07 DNA extraction

BEXS 12.Your morning back from the bench.

By hand, every batch of DNA means columns, a centrifuge and an hour of pipetting. BEXS 12 takes twelve blood samples to purified DNA on its own.

Lift the door.Load the deck once.

  • One Tip & Tube RackThe deep well plate, tips, bead tubes and elution tubes, set out in one go.
  • 7 buffers dispensed for youThe bottles hang on a barcoded holder; the instrument doses every step.
  • Touchscreen and barcode readerChoose the program, scan, start. Nothing to type.

Twelve magnetic rods.No centrifuge.

  • DNA binds the beadsCoated magnetic particles hold the DNA in the presence of chaotropic salts.
  • The rods carry the beadsMagnetised, they collect the beads well to well. Fast rotation resuspends them.
  • Wash, then eluteThe platform steps the plate row by row under the rods.

Close. Start “Blood 250”.Forty minutes, twelve eluates.

  • 250 µl of blood in5 to 10 µg of DNA out, A260/A280 about 1.8.
  • CE certified as a systemWith the BEXS 12 Ready DNA Bead Kit and inno-train SuBiTo, Ready Gene and FluoGene kits.
  • Ready for PCRThe eluate goes straight into your downstream test.

The kit

One box.
Fifteen mornings of DNA.

Everything a run uses comes counted out: hang the buffers in, set down a plate and the tips, and the box covers 180 samples, twelve at a time.

The BEXS 12 Ready DNA Bead Kit: seven buffer bottles on a yellow barcoded plate, magnetic bead suspension, deep well plates, tips and elution tubes
  • 7buffers on one barcoded plateLoad all seven in one move. No mixing, no measuring.
  • 15deep well platesOne plate per run of twelve samples.
  • 192tipsTwelve per run, already in the box.
  • 384elution tubesYour DNA ends up here, ready for PCR.
  • Beadsmagnetic suspensionThey catch the DNA from 250 µl of blood.

Which fits your lab

Same DNA quality.
Three ways to get it.

  • Inside the BEXS 12: twelve magnetic rods over a deep well plate

    You extract every day.

    BEXS 12, automated

    12
    samples a run
    40 min
    walk-away time
    0
    centrifuge steps

    Instrument and kit for 180 samples

  • Twelve tubes on a magnetic bead stand

    A few samples, no instrument.

    Ready DNA Bead Kit, by hand

    12
    samples at a time
    30 min
    at the bench
    1
    magnetic stand

    Bead stand and kit for 50 samples

  • A spin column with its silica membrane in a collection tube

    Cells or tissue, not only blood.

    Ready DNA Spin Kit

    3
    sample types
    30 min
    at the bench
    1
    microcentrifuge

    Kit for 50 samples

Spare deep well plates, elution tubes, bottle spikes and tubing for the BEXS 12 are available separately.

08 Component processing

Collect. Separate.
Seal. Connect.

Six machines for the component lab, from the donor chair to the sterile weld. Extractors and a tube welder from Lmb Technologie, Schwaig, Germany.

NOVOmatic, SuperNOVA and LUXOmatic V2 component extractors, a WELDmatic sterile tube welder, a tube sealer and a blood collection monitor, shown together
  1. NOVOmatic single press extractor

    Single press extractor

    NOVOmatic

    Clever, simple, precise. A motor driven double press for plasma and additive solution transfer, self-adjustable sealing heads and a colour touchscreen.

    Balances
    2, a 3rd optional
    Sealing
    5 heads, 0.5 to 5 s
    Red cell sensing
    6 sensors in a row, 4 in the sealing head
    Size
    551 × 357 × 415 mm, about 31 kg
  2. SuperNOVA component extractor

    Single press, more sensing

    SuperNOVA

    A large colour touchscreen and two-way data transfer on LAN, with sensing that follows every bag to the last millilitre.

    Balances
    4
    Sealing
    Up to 5 clamps, 2 to 5 s
    Red cell sensing
    16 sensors in a row, 4 in the sealing heads
    Size
    564 × 920 × 554 mm, 30 kg
  3. LUXOmatic V2 dual press extractor

    Dual press

    LUXOmatic V2

    Two independent separation places in one footprint, so two bags from one centrifuge basket are separated side by side, from paediatric bags to heavy duty runs.

    Separation places
    2
    Balances
    8
    Sealing
    8 self-adjustable clamps, plus 2 non-sealing
    Red cell sensing
    4 adaptive optical and 6 HCT sensors per side
  4. WELDmatic sterile tube welder

    Sterile connection

    WELDmatic

    A sterile medical PVC tube welder. No blade, no copper wire and no disposables, with a patented automatic opening at the connection point.

    Tubing
    3.9 to 4.8 mm outer diameter
    Weld time
    15 to 21 s
    Strength
    100+ N tensile, dry-dry and wet-dry
    Clean air
    HEPA and carbon filters, UV sterilizer

  5. SEALmatic M tube sealer with handheld sealing head and cable

    Tube sealing

    Tube sealer

    A bench unit with a handheld sealing head. It seals blood bag tubing closed at the bag, or along the line to make segments for testing.

  6. Blood collection monitor

    Collection

    Blood collection monitor

    It weighs the bag and rocks it gently during donation, so blood and anticoagulant mix evenly, and stops the draw at the set volume.

CollectionComponent separationTube sealingSterile connection

Demotek quintuple top and bottom blood bag set with an in-line red cell filter Quadruple blood bag set, CPD and SAG-M Quadruple top and bottom blood bag set Quadruple blood bag set with an in-line red cell filter Paediatric blood bag set with an in-line whole blood filter

09 Blood bags and filters

Demophorius.Where every component begins.

Skin bacteria in the first millilitres, white cells left in stored red cells, an open step during separation: most problems with a blood component start in the bag it was collected in. Demophorius bag systems stay closed from the needle to the last satellite bag.

Triple and quadruple.The everyday sets.

  • CPDA-1 or CPD with SAG-MTriple and quadruple bag systems for 350 and 450 ml collections.
  • Sampling device and needle guardThe first flow goes to a pouch for the test tubes, not into the unit. The 16G needle goes back into its guard after donation.
  • 5 day platelet bagIncluded in the triple and quadruple sets.

Top and bottom.Plasma up, red cells down.

  • Two outlets on the primary bagAfter centrifugation the plasma leaves by the top and the red cells by the bottom.
  • The buffy coat staysIt remains in the primary bag, ready to be pooled for platelets.
  • Made for extractorsTriple and quadruple sets for automatic component extractors with top and bottom programmes.

In-line filtration.White cells stay behind.

  • Red cell filter in the setRed cells are leucoreduced before storage, without opening the system.
  • Why it mattersWhite cells in transfused blood are linked to febrile reactions, HLA immunisation and CMV transmission.
  • Three components from one bagThe Demotek quintuple set gives plasma, a platelet concentrate and leucoreduced red cells from one collection of 450 ml.

Filters, poolingand paediatric.

  • Bedside and laboratory filtersFor a unit of whole blood or red cells, and for platelets.
  • Buffy coat poolingA 1500 ml pooling bag with an in-line platelet filter and a sampling bag.
  • Paediatric bagsSmall satellite bags of 150 ml, one set with an in-line whole blood filter.

Blood bag systems

Pick the set
for your process.

DEHP PVC bag systems from Demophorius Healthcare, each supplied with a 16G needle and the anticoagulant shown.

  • Triple bag

    Triple bag

    CPDA-1 with a 16G needle, sampling device and needle guard. Includes a 5 day platelet bag.

    • 350 ml
    • 450 ml
  • Quadruple bag

    Quadruple bag

    CPD/SAG-M with a 16G needle, sampling device and needle guard. Includes a 5 day platelet bag.

    • 450 ml
  • Top and bottom, triple

    Top and bottom, triple

    CPD/SAG-M, with a sampling device and a needle guard, for top and bottom separation.

    • 350 ml
    • 450 ml
  • Top and bottom, quadruple

    Top and bottom, quadruple

    CPD/SAG-M with a sampling device, needle guard and a 5 day platelet bag.

    • 450 ml
  • In-line red cell filter

    In-line red cell filter

    Quadruple bags with an in-line leukoreduction filter for red cells, standard or top and bottom.

    • Standard · 450 ml
    • Top and bottom · 450 ml
  • Quintuple, top and bottom

    Quintuple, top and bottom

    The Demotek set: in-line red cell filter, sampling device and needle guard.

    • 450 ml
  • Paediatric bag, CPDA-1

    Paediatric bag, CPDA-1

    1 × 450 ml and 4 × 150 ml with a sampling device and needle guard, or 450, 450, 350 ml and 3 × 150 ml with an in-line whole blood filter.

    • 150 ml satellites
  • Bedside leukoreduction filterFor one unit of whole blood or red cells, or for platelets.
  • Laboratory leukoreduction filterFor red cells in a DEHP PVC bag, or for platelets in a 1 × 600 ml DEHP PVC bag.
  • Buffy coat pooling system1 × 600 ml (DEHP) or 1500 ml (BTHC) bag, 150 ml sampling bag and an in-line platelet filter.

Line drawings are the manufacturer’s artwork, matched to each set by the number of bags and the filter.

Filters, pooling and paediatric

Bedside, laboratory
and small volume.

ProductFeatures
Leukoreduction filter, bedsideOne unit of whole blood or red cells
Leukoreduction filter, bedsideFor platelets
Leukoreduction filter, laboratoryFor red cells, DEHP PVC bag
Leukoreduction filter, laboratoryFor platelets, 1 × 600 ml DEHP PVC bag
Buffy coat pooling system1 × 600 ml (DEHP) or 1500 ml (BTHC), 150 ml sampling bag, in-line platelet filter, 1 segment
Buffy coat pooling systemAs above, with 7 segments
Paediatric bag, CPDA-11 × 450 ml and 4 × 150 ml, sampling device, needle guard
Paediatric bag, CPDA-1450, 450, 350 ml and 3 × 150 ml, in-line whole blood filter

Demophorius Healthcare, Limassol, Cyprus.

10 Transfusion instruments

Everything a transfusion service needs.From one manufacturer.

Imperial Biotech builds the instruments around every donation, from the donor couch to the autoclave. Twenty-seven of them, made in Greater Noida.

The range

Twenty-seven instruments.
Collection to culture.

Imperial Biotech is ISO 13485:2016 and CE certified. Models and figures are as printed in the 2025 catalogue.

Donors and collection

  • Blood donor couch
    01Blood donor couchIDC-01 · 02 · 03

    One, two or three geared motors on a remote. Head down and legs up in one move, so blood returns to the brain in a vasovagal reaction.

    60 or 150 kg · lockable castors
  • Portable donor chair
    02Portable donor chairIPDC-01

    A light, foldable stainless steel chair for blood camps, with two adjustable arm rests and washable covers.

    Folds for carrying
  • Phlebotomy chair
    03Phlebotomy chairSampling chair

    Gas-jack height, a back rest that moves from 0 to 90° and two blood test splints that lock in any position.

    SS-304 frame · 130 kg dynamic load
  • Blood collection monitor
    04Blood collection monitorIBCM-01 · 02

    Rocks the bag at 12 ± 2 RPM to mix blood with anticoagulant, weighs to ± 1 ml and clamps the tube at the set volume.

    1 to 999 ml · 10 h battery on IBCM-02
  • Blood component balance
    05Blood component balanceISPB-01

    A portable balance with tare for the bag and auto calibration, for whole blood, packed cells, plasma and platelets.

    ± 1 g · battery back-up
  • Double pan balance
    06Double pan balanceIDPB-02

    Balances the opposite buckets before the spin, with tare for the bags and calibration from the panel.

    6 kg · ± 1 g
  • Tube stripper
    07Tube stripperITS-01

    A stainless steel body, a Delrin roller and a plastisol grip, to strip the tubing into segments.

    EN ISO 13485

Separation

  • Refrigerated centrifuge
    08Refrigerated centrifugeIRC-04 · 06 · 08 · 12

    A colour touch screen, programmable memory with a tamper-proof lock and metal buckets for 4 to 12 bags. Speed is held within 10 RPM of the set value.

    Up to 4800 RPM · −20 to +40 °C
  • Plasma extractor
    09Plasma extractorIPE-01 · 02

    A spring-loaded front plate presses the spun bag so the plasma flows into the transfer bag. Manual, or motor clamping with an infrared sensor.

    Acrylic pressure plate
  • Blood bag tube sealer
    10Blood bag tube sealerITS-02 · 03

    Seals each tube by radio frequency in about two seconds. The ITS-03 runs on battery for about 800 seals.

    40.68 MHz · 4.5 to 5.25 kg

Storage

  • Blood bank refrigerator
    11Blood bank refrigeratorIBBR-100 to 600

    Whole blood at a preset 4 ± 1 °C, stainless steel drawers, a 7-day chart recorder and alarms for temperature, power, door and sensor.

    200 to 1000 l · 100 to 600 bags
  • Platelet incubator and agitator
    12Platelet incubator and agitatorIPIA-01 · 02

    Platelets at 21 ± 1 °C on a motorised slide that keeps them moving at 70 RPM, day and night.

    48 or 96 bags · 8 or 16 trays
  • Plasma freezer
    13Plasma freezerIPF-300 to 1000

    Plasma bags and samples down to −40 °C, with a stainless steel inner door and 120 mm CFC-free insulation.

    300 to 1000 l · 4 to 8 drawers
  • Ultra low deep freezer
    14Ultra low deep freezerIDPF-150 to 600

    Down to −80 °C for plasma and test samples, upright or chest, with a 7-day chart recorder.

    150 to 600 l · 2 to 6 drawers
  • Plasma thawing and cryo baths
    15Plasma thawing and cryo bathsIPTB-01 · ICB-01

    A pump circulates the water around a stainless steel basket. The thawing bath runs at +37 °C, the cryoprecipitate bath at +4 or +37 °C.

    12 bags · ± 0.2 °C
  • Reagent refrigerator
    16Reagent refrigeratorILR-320 to 1000

    Reagents, kits and samples at 2 to 8 °C, with forced air circulation, a lockable glass door and internal light.

    320 to 1000 l · 1 or 2 doors

Serology and laboratory

  • Gel card centrifuge
    17Gel card centrifugeIGCC-01

    Spins 12 gel cards at 1600 RPM, with programmable runs and a lid that locks until it stops.

    0 to 99 min
  • Gel card incubator
    18Gel card incubatorIGCI-01

    Warms 24 gel cards at 37 °C behind an acrylic door, with a 15 minute timer and alarm.

    25 to 40 °C
  • RH view box
    19RH view boxSlide viewer

    Brings an RH slide to 37 to 39 °C in under two minutes, under soft, glare-free light. Tilt it with the knob to read.

    Built-in temperature control
  • VDRL shaker
    20VDRL shakerIVS-01

    Gentle, concentric motion for syphilis test cards, with a spring clamp on the carrier.

    13 × 13 in · 70 to 200 RPM
  • Water bath
    21Water bathIWB-01

    A stainless steel 304 tank with the heater inside, for fast and even warming.

    30 to 100 °C
  • Laminar air flow
    22Laminar air flowILAF-2 · 3 · 4

    A HEPA filter at 99.997 % down to 0.3 µm, a pre-filter, UV tube and a stainless steel work top.

    2, 3 or 4 ft
  • Biosafety cabinet
    23Biosafety cabinetIBBC-101 · 102

    Class II, type A2 or B2. An inflow air curtain protects the user, HEPA-filtered downflow the sample, filtered exhaust the room.

    3 or 4 ft
  • Hot air oven
    24Hot air ovenIHAO-01

    Dry heat sterilisation up to 300 °C, with a PID controller, timer and fan-forced air.

    +5 to 300 °C · 3 shelves
  • Incubator
    25IncubatorINC-01

    Double walled, for bacterial culture, with a glass inner door to look in without losing heat.

    25 to 70 °C · ± 1 °C
  • Autoclave
    26AutoclaveIAC-01

    Steam sterilisation with vacuum and non-vacuum cycles, five or more stored programs and a panel that records every batch.

    121 to 138 °C · up to 1000 l
Nigale NGL XCF 3000 blood component separator

Apheresis · Nigale

NGL XCF 3000.
Platelets from one donor.

A patient with dengue, or on chemotherapy, needs a full dose of platelets. From whole blood that means pooling the platelets of four to six donations. The XCF 3000 collects the dose from one donor, in one sitting, and gives the rest back.

  • One needleBlood is drawn and returned through the same needle, in one arm.
  • Spun into layersThe centrifuge, up to 5500 rpm, sends the red cells to the wall of the bowl; the platelets settle in a thin layer inside them, plasma at the core.
  • Only the platelets are keptThe platelet layer goes to the collection bag. Red cells and plasma go back to the donor, and the cycle repeats until the dose is reached.
  • Double dose, leukoreducedA protocol for two doses of leukoreduced platelets from one donor. Plasma too.
  • Easy on the donorThe pump flow follows the donor, for comfort. Alarms with the likely cause on the touch screen.
The NGL XCF 3000 centrifuge chamber and valves
Collects
Platelets, double-dose leukoreduced platelets, plasma
Centrifuge
4800 or 5500 rpm
Size
57 × 36 × 45 cm, about 36 kg, ready for mobile collection
Standard
Built to the EDQM standard

Nigale P-2000 sets

One set per donor.
Bowl to bag, in one piece.

Each set comes pre-connected and sterile: the centrifuge bowl, the lines, the needle and the bags. It goes onto the machine in one piece, which keeps loading simple and the risk of contamination low.

  1. Platelets

    A full dose from one donor, in one collection bag.

    Bowl · lines · needle · collection and sample bags
  2. Double-dose platelets

    Two leukoreduced doses from one donor, in one sitting.

    Bowl · lines · needle · collection and sample bags
  3. Plasma

    Plasma into a bottle, with a sample bag for the tests.

    Bowl · lines · needle · plasma bottle · sample bag

11 Our partners

Five manufacturers.
One partner in Chennai.

USAS Medical Enterprise brings these makers’ products to hospitals, blood banks and laboratories, with the information you need to choose.

  1. FluoQube, with a FluoGene plateBEXS 12BEXS kitHLA NGStype kitDaraExDara-PCDaraEx plus inno-trainKronberg, GermanyHLA and blood group typing, from DNA extraction to NGS. FluoQube and FluoGene, NGStype, Ready Gene, BEXS 12 and DaraEx.See products
  2. Lumilight PelhamItalyLumilight photopheresis: one bag, one thin layer, the same dose for every cell.See products
  3. NOVOmatic, SuperNOVA, LUXOmatic, WELDmatic, tube sealer and blood collection monitor Lmb TechnologieSchwaig, GermanyComponent processing, from separation to sterile welding. NOVOmatic, SuperNOVA, LUXOmatic, WELDmatic, a tube sealer and a collection monitor.See products
  4. Quintuple top and bottom set with filterQuadruple set with filterPaediatric set Demophorius HealthcareLimassol, CyprusBlood bag systems, from paediatric sets to quintuple top and bottom sets, with in-line filters.See products
  5. Blood bank refrigeratorUltra-low freezerLaminar air flowAutoclaveRefrigerated centrifugeNigale apheresis separatorDonor couchPlasma thawing bathGel card centrifugeTube sealerBlood collection monitor Imperial BiotechGreater Noida, IndiaEverything a transfusion service needs, from the donor couch to the autoclave, and the Nigale apheresis separator.See products

Coverage

South India,
from Chennai.

Based in Mogappair, Chennai, serving hospitals and laboratories across south India.

About USAS

We bring specialist transfusion, immunology and genomic technology to the hospitals and laboratories of south India, and we install and service what we supply.

U. S. Automations & Services, trading as USAS Medical Enterprise. Chennai.

What we bring

Across the transfusion chain,
from the donor’s chair to the patient.

  1. CollectDonor couches, collection monitors and the Nigale apheresis separatorImperial Biotech
  2. SeparateLUXOmatic, NOVOmatic, SuperNOVA and WELDmaticLmb Technologie
  3. StoreBlood bag systems and in-line filtersDemophorius Healthcare
  4. ExtractDNA from blood, ready for typinginno-train
  5. TypeHLA serology plates and DaraExinno-train
  6. GenotypeFluoQube, FluoGene and Ready Geneinno-train
  7. SequenceNGStype for HLA and red cell antigensinno-train
  8. TreatLumilight extracorporeal photopheresisPelham
  • 8steps of the chain covered
  • 10product areas, from cell therapy to DNA
  • 5manufacturers’ ranges

How we work

One partner, from the first question
to the last service visit.

  1. Advise

    Help in choosing the right system, with the manufacturer’s data in hand.

  2. Supply

    Authorised channel partner for leading global and Indian brands.

  3. Install

    On-site set-up by our engineers.

  4. Service

    Warranty support, spare parts and annual maintenance contracts.

Who we serve

The teams behind
every transfusion.

The people we work with every day, across hospitals and laboratories.

  • Hospitals and transfusion medicine departments
  • Blood centres and component laboratories
  • HLA, transplant and molecular laboratories
  • Haematology, oncology and transplant units
  • Research and academic laboratories

Find us

Come and see us
in Mogappair, Chennai.

No. C-3, Karnan Street, Then Thirupathi Nagar,
Mogappair, Chennai, Tamil Nadu 600037

Get directions

Call+91 97890 52767 Writeinfo@us-automations.com